產品詳情
簡單介紹：
重組鼠脂肪酸結合蛋白1蛋白與其它公司提供的重組蛋白不同，rMuFABP1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠脂肪酸結合蛋白1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠脂肪酸結合蛋白1蛋白
| Synonyms | 14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP |
| Species | Murine |
| Accession | P12710 |
| GeneID | 14080 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠脂肪酸結合蛋白1蛋白Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠脂肪酸結合蛋白1蛋白Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose. |
| Endotoxin | Less than 1 EU/μg of rMuFABP1 as determined by LAL method. |
| Reconstitution | 重組鼠脂肪酸結合蛋白1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠脂肪酸結合蛋白1蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Chmurzynska A. 2006. J Appl Genet. 47:39-48. 2. Weisiger RA. 2002. Mol Cell Biochem. 239:35-43. 3. Chen SH, Van Tuinen P, Ledbetter DH, et al. 1986. Somat Cell Mol Genet. 12:303-6. 4. Weickert MO, Loeffelholz CV, Roden M, et al. 2007. Am J Physiol Endocrinol Metab. 293:E1078-84. 5. Bassuk JA, Tsichlis PN, Sorof S. 1987. Proc Natl Acad Sci U S A. 84:7547-51. 6. He Y, Yang X, Wang H, et al. 2007. Biochemistry. 46:12543-56. |
| Background | The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. Fatty acid-binding protein 1 (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP1 binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP1 shares 84 % and 94 % a.a. sequence identity with human and rat FABP1. |
